Relation between Ion Accumulation of Salt-Sensitive and Isolated Stable Salt-Tolerant Cell Lines of Citrus aurantium

Abstract

Four selected NaCl-tolerant cell lines of Sour orange (Citrus aurantium) were compared with the nonselected cell line in their growth and internal ion content of Na(+), K(+), and Cl(-) when exposed to increasing NaCl concentrations. No difference was found among the various NaCl-tolerant cell lines in Na(+) and Cl(-) uptake, and all these cell lines took up similar or even larger amounts of Na(+) and Cl(-) than the NaCl-sensitive cell line. Exposure of cells of NaCl-sensitive and NaCl-tolerant lines to equal external concentrations of NaCl, resulted in a greater loss of K(+) from the NaCl-sensitive cell line. This observation leads to the conclusion that growth and ability to retain high levels of internal K(+) are correlated. Exposure of the NaCl-tolerant cell lines to salts other than NaCl resulted in even greater tolerance to Na(2)SO(4), but rather poor tolerance to K(+) introduced as either K(2)SO(4) or KCl; the latter has a stronger inhibitory effect. The NaCl-sensitive cell line proved to be more sensitive to replacement of Na(+) by K(+). Analyses of internal Na(+), K(+), and Cl(-) concentrations failed to identify any particular internal ion concentration which could serve as a reliable marker for salt tolerance.