Assessment of lipid peroxidation and serum vitamin E concentration in dogs with immune-mediated hemolytic anemia

Abstract

Objective - To determine plasma malondialdehyde (MDA) and serum vitamin E concentrations in dogs with immune-mediated hemolytic anemia (IMHA) and healthy control dogs. Sample Population - Serum and plasma samples from 36 dogs with IMHA and 40 healthy control dogs. Procedure - Blood samples were collected from all study dogs. Plasma MDA concentrations were measured by use of a commercial colorimetric assay, and serum vitamin E concentrations (α-, γ-, and δ-tocopherol concentrations) were measured via high-performance liquid chromatography. Results - Plasma MDA concentrations were significantly higher in the dogs with IMHA than in the control dogs. Compared with control dogs, serum (α-, γ-, and δ -tocopherol concentrations were significantly lower in the IMHA-affected dogs. Conclusions and Clinical Relevance - Results indicated a state of oxidative stress and reduced antioxidant reserve in dogs with IMHA; this finding provides support for further investigation of the potential benefits of antioxidant treatment in dogs with this disease.