Kinetic evidence of an on‐pathway intermediate in the folding of lysozyme

Abstract

By means of a kinetic test, it was demonstrated that one of the folding intermediates ( I α ) of hen lysozyme with α‐domain folded and β‐domain unfolded is on the folding pathway under the classical definition. l α folds to the native ( N ) state directly (unfolded ( U ) ⟺ I α ⟺ N ) without having to unfold to U and then refold to N through alternative folding pathways as in I α ⟺ U ⟺ N .