Investigation of phenotypic and genotypic characteristics of isolated Vibrio anguillarum strains in rainbow trout cage farms in Kahramanmaras city of Turkey

Abstract

The purpose of this study is to isolate and examine the phenotypic and genotypic characteristics of the Vibrio anguillarum strains in rainbow trout cultured in floating cage farms in the province of Kahramanmaraş, Turkey. In June 2014, liver, spleen, kidney, and intestines samples of farmed rainbow trout (250-300 g) were collected from different fish farms in the region. Two methods were used to identify V. anguillarum in the samples. The Biolog System was used investigate the presence of V. anguillarum obtained from rainbow trout samples and examine the phenotypic and biochemical characteristics for the identification of V. anguillarum. The polymerase chain reaction technique with a specific primer was used to validate different V. anguillarum strains, according to phenotypic and biochemical characteristics. The results indicated that all of the isolated samples exhibited V. anguillarum. Sequence analysis was performed on the V. anguillarum strains obtained from the samples, and the corresponding data were analyzed. Compared to the culture method which required three weeks to identify V. anguillarum, the PCR technique provided rapid results in a matter of minutes