Screening of antimicrobial lactic acid bacteria against bovine mastitis

Abstract

Bovine mastitis is costly infectious disease of dairy cattle, being responsible for significant economic losses all over the world. Also, mastitis has troubled about resistance to antibiotics. The purpose of this study was to screen a novel antimicro-bial strain from various sources (raw milk and feeds (from farm of Paju, Dangjin, and Hwasung), commercial milk, Korean traditional fermented foods, and chicken feces). The isolate was screened using triple agar layer method and deferred method was used for confirmation of antimicrobial effect. Seventy six of isolates were screened using triple agar layer method. In these strain, 42 isolates were shown a broad spectrum of antimicrobial activity against mastitis pathogens. Espe-cially, fourteen isolates were shown over 20 mm inhibition zone against S. aureus ATCC 25923. These results suggest that these novel antimicrobial strains could be used for the alternative of antibiotics.