In vitro seed propagation of Dendrobium moschatum as influenced by different plant growth regulators

Abstract

This study aimed to develop an effective in vitro protocol for germinating and multiplying Dendrobium moschatum seeds by identifying optimal concentrations of plant growth regulators for shoot proliferation and root formation. Five different MS media formulations were utilized, including a control (M1) and those supplemented with varying concentrations of kinetin and NAA (M2, M3, M4) and BAP (M5). Results showed significant variation in seed germination time across media, with the longest duration observed in M4(76 days) and the shortest in M1(55 days). Protocorm formation and plantlet development were quickest in M1, while M3and M5exhibited the longest duration. The initiation of leaves or shoots occurred earliest in M3(103 days) and latest in M5(149 days). Notably, leaf and plantlet growth differed between three- and five-month intervals, with M3demonstrating optimal growth over the longer term. These findings underscore the importance of carefully balanced plant growth regulator concentrations in achieving successful seed germination and subsequent growth in D. moschatum.