FLAVONOID GLYCOSIDE COMPOUND FROM TOMBILI SEED (Caesalpinia bonducella) AND ITS ANTIOXIDANT ACTIVITY

Abstract

Caesalpinia bonducella, known as ‘tombili’ belongs to the family of Fabaceae. Tombili has been empirically used as traditional medicine. In a previous study, phytochemical screening showed that tombili seed contained alkaloid, flavonoid, terpenoid, and tannin compounds. This research aimed to isolate an antioxidant compound from tombili seed extract. Fractionated using n-hexane, ethyl acetate, and methanol as a solvent. All fractions were tested for their antioxidant potential. The ethyl acetate fraction gave a better antioxidant potential (IC50 86.153±4.22 ppm) than it has purified. Characterization of an isolated compound using various spectroscopies data, including UV, FTIR, 1D-NMR, 2D-NMR, and LCMS/MS. The structure of the isolated compound was suggested as 7-(β-D-Glucopyranosyloxy) 5-hydroxy-2-(4-hydroxyphenyl)-6-methoxy-4H-1-benzopyran-4-one (homoplantaginin). The IC50 value of the isolated compound was 40.53 ±3.13 ppm, indicating the isolated compound has potent antioxidant activity.