Influence of different wastewater treatment technologies on genotoxicity and dioxin-like toxicity in effluent-exposed fish

Abstract

Background: In situ exposure of rainbow trout up- and downstream of differently equipped wastewater treatment plants (WWTPs) and subsequent analyses of micronuclei frequencies and hepatic EROD activities were used to evaluate the impact of the effluents on fish health. Two of the facilities (WWTPs A and B) were conventional treatment plants. WWTP C has been equipped with a powdered activated carbon stage. Here, analyses were conducted prior and subsequent to this upgrade. Results: Differences did not only occur when comparing conventional (WWTPs A, B and C prior to the upgrade) and advanced treatment (WWTP C after the upgrade), but also between the conventionally equipped WWTPs. There was no indication for genotoxic effects or pollution-related EROD induction in fish exposed at WWTP A. In contrast, trout exposed at WWTP B expressed strong reactions. However, here, adverse reactions were also observed in fish kept upstream. Similar observations were made for EROD activities in fish exposed at WWTP C prior to the upgrade, whereas genotoxic effects could only be seen in trout kept downstream of this effluent. Upgrading of WWTP C resulted in a significant reduction of both genotoxic effects and EROD levels. Conclusions: The results show financial investments in advanced wastewater treatment to be beneficial for aquatic ecosystems, especially when conventional technologies do not sufficiently remove pollutants. Yet, negative impacts of effluents on aquatic organisms can, under certain conditions, also be avoided by conventional treatment. Therefore, we recommend deciding on the necessity and the type of WWTP upgrading on a case-by-case basis.