Secuencias de inserción IS6110 e IS1081 en cepas de Mycobacterium bovis provenientes de bovinos beneficiados en la Región Metropolitana

Abstract

Secuencias de inserción IS6110 e IS1081 en cepas de Mycobacterium bovis provenientes de bovinos beneficiados en la Región Metropolitana PATRICIO RETAMAL M. 1 , M. ANGÉLICA MARTÍNEZ T. 2 y PEDRO ABALOS P. 1 Insertion sequences IS6110 and IS1081 present in strains of Mycobacterium bovis from cattle slaughtered at the Metropolitan Area, Chile Tuberculosis remains one of the major world health problems. Several studies have established the usefulness of insertion sequence (IS) IS6110 and IS1081 fingerprinting for the identification and typing of strains from the Mycobacterium tuberculosis complex. Both sequences are specific of bacteria belonging to this complex, but the number of copies can be variable among different M. tuberculosis and M. bovis strains. The aim of our study was to survey the presence of the insertion sequences IS6110 and IS1081 in nine strains of Mycobacterium bovis isolated from the mediastinal lymph nodes of cattle slaughtered in Santiago, Chile. All the strains contained IS1081, whereas only seven of them contained the IS6110 sequence. These results suggest the potential use of IS1081 for the differentiation by RFLP analysis of M. bovis strains. IS6110 may constitute an additional genetic marker for typing strains containing this element. It is recommended the characterization of this insertion sequences with genotyping assays, with higher sample sizes, more animal species and from different geographical areas to obtain useful epidemiological information in further control programs of the disease.