A New Sterol From Sporoderm-Broken Ganoderma sinense Spores and Its Anticancer Activity

Abstract

A new sterol, ganodermaside E (1), and 4 known sterols, (22E,24R)-3β,5α-dihydroxyergosta-7,22-dien-6-one (2), (22E,24R)-3β,5α,9α-trihydroxyergosta-7,22-diene-6-one (3), (22E,24R)-ergosta-7,9(11),22-triene-3β,5α,6β-triol (4), and (22E,24R)-ergosta-7,22-diene-3β,5α,6α-triol (5), were isolated for the first time from the sporoderm-broken spores of Ganoderm sinense Zhao, Xu et Zhang. Their structures were determined by spectroscopic techniques such as nuclear magnetic resonance spectroscopy and mass spectrometry. Furthermore, all the compounds were evaluated for their in vitro cytotoxicity and migration inhibition on human non-small-lung cancer A549 cells. Compound 1 exhibited cytotoxicity with a half-maximal inhibitory concentration value of 21.12 ± 1.46 µM. Compound 5 exhibited the strongest and most significant antimetastatic activity at concentrations of 100 and 200 µM.