Studies on inhibition of Morinda citrifolia leaf extract (Morinda citrifolia L) against the growth of Aeromonas hydrophilla in vitro

Abstract

Morinda citrifolia leaf contains active compounds that function as antibacterial substances, namely saponin, flavonoid, polyphenol, tannin and triterpen. The purpose of this research to discover the effectiveness of the Morinda citrifolia leaf extract in order to inhibit the growth of the bacteria Aeromonas hydrophilla as a cause of Motile Aeromonas Septicemia disease (MAS) on freshwater fish. This research is experimental research laboratory in vitro. The method used as an antibacterial test is a soft diffused method paper disc. The proportion of Morinda citrifolia leaf extract used in this research are 7.5%, 15%, 30%, 60% and negative control (ethanol 96%). The results of the study showed that Morinda citrifolia leaf extract is significantly (p<0.05) effective in inhibiting the growth of the bacteria Aeromonas hydrophilla. It shows that higher proportions of Morinda citrifolia leaf extract then inhibit zone formed when increasingly wide.