Development and characterization of polymorphic microsatellite loci for spiny-footed lizards, Acanthodactylus scutellatus group (Reptilia, Lacertidae) from arid regions

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Abstract

Background: Spiny-footed lizards constitute a diverse but scarcely studied genus. Microsatellite markers would help increasing the knowledge about species boundaries, patterns of genetic diversity and structure, and gene flow dynamics. We developed a set of 22 polymorphic microsatellite loci for cross-species amplification in three taxa belonging to the Acanthodactylus scutellatus species group, A. aureus, A. dumerili/A. senegalensis and A. longipes, and tested the same markers in two other members of the group, A. scutellatus and A. taghitensis. Results: Amplifications in A. aureus, A. longipes and A. dumerili/A. senegalensis were successful, with markers exhibiting a number of alleles varying between 1 and 19. Expected and observed heterozygosity ranged, respectively, between 0.046-0.893 and 0.048-1.000. Moreover, 17 and 16 loci were successfully amplified in A. scutellatus and A. taghitensis, respectively. Conclusion: These markers are provided as reliable genetic tools to use in future evolutionary, behavioural and conservation studies involving species from the A. scutellatus group.

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Lopes, S. C., Velo-Antón, G., Pereira, P., Lopes, S., Godinho, R., Crochet, P. A., & Brito, J. C. (2015). Development and characterization of polymorphic microsatellite loci for spiny-footed lizards, Acanthodactylus scutellatus group (Reptilia, Lacertidae) from arid regions. BMC Research Notes, 8(1). https://doi.org/10.1186/s13104-015-1779-3

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