Hyaluronic Acid /Carboxymethyl Chitosan Embedded Gold Nanoparticles Modulate High Fructose Diet Induced Diabetes Changes in Rat

Abstract

In this current work, hyaluronic acid (HA)/carboxymethyl chitosan (CMCTs) blend, embedded with definite amount of gold nanoparticles AuNPs, are utilized to mitigate diabetic parameters in experimental model of diabetes in rats. AuNPs was produced thru Microwave radiation technique, followed by characterization using state of art analysis; UV and TEM. Afterward, 50”male albino rats" were divided into two main groups: Group one "n=10 rats" fed on the healthy diet (-ve control). The 2nd group fed on the high fructose diet (HFD) for 4 weeks to induce diabetes. To this end, the 2nd group, is further divided into four sub-groups. The 1st one (G2) received only HFD (+ve control), 2nd,3rd and 4th subgroups (G3, G4 and G5) received orally; mixture of both HA and CMCTS in different ratios (1:3; 1:1; 3:1 respectively) embedded all with AuNPs (0.01M), in a dose of 2 mg/kg body weight /day for 4 weeks. In addition, the effect of polymers blends with AuNPs on Streptococcus faecalis, Escherichia coli and Aspergillus fumigatus was also evaluated. All treated groups (G3, G4, G5) decreased diabetic biomarkers Fasting blood sugar (FBS), fasting insulin, glycosylated haemoglobin (HbA1C), "Homeostasis model assessment of insulin resistance (HOMA-IR)" index; “homeostasis model assessment of βcell function (HOMA-β)” compared with the +ve control. Moreover, the mixture of HA/CMCTS (1:3) adorned AuNPs was more potent than the other used ratios from the polymer combination, in attenuation of diabetic intricacy, however, the antimicrobial activity for all mixture was effective but almost the same. Furthermore, all treated subgroups disclosed normal histopathological pancreatic cell structures.