DMPO spin trapping of superoxide anion in strong alkaline DMSO solution

Abstract

Superoxide anion (O2•-), generated in a strong alkaline DMSO solution and detected by direct 77 K EPR, was trapped with 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) and the resultant nitroxide radical, DMPO/O2•-, has been found to be sufficiently stable for the subsequent EPR detection.