Determination of sulfonamides and trimethoprim using high temperature HPLC with simultaneous temperature and solvent gradient

Abstract

A fast HPLC method for the analysis of eight selected sulfonamides (SA) and trime-thoprim has been developed with the use of high temperature HPLC. The separation could be achieved in less than 1.5 min on a 50 mm sub 2 μm column with simultaneous solvent and temperature gradient programming. Due to the lower viscosity of the mobile phase and the increased mass transfer at higher temperatures, the separation could be performed on a conventional HPLC system obtaining peak widths at half height between 0.6 and 1.3 s. © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.