Glutathione S -Transferase Genes Differently Expressed by Pathogen-Infection in Vitis flexuosa

Abstract

See, stats, and : https :// www. researchgate. net/ publication/ 296621680 Glutathione -Transferase Expressed -Infection flexuosa Article DOI : 10 . 9787 / PBB . 2016 . 4 . 1 . 061 CITATIONS 3 READS 80 3 , including : Some : management Seon - ae Yeungnam 19 SEE Haekeun Yeungnam 75 SEE All . The . ABSTRACT Glutathione S - transferase (GST) genes from transcripts of Vitis flexuosa leaves infected with Elsinoe ampelina were characterized and analyzed for their expression using primers based on specific regions . Comparison of deduced amino acid sequences from GST transcripts of V . flexuosa showed that the score of the deduced amino acid identity ranged from 43 . 38% (Vf GST26625 and Vf GST774) to 6 . 67% (Vf GST13892 and Vf GST774) . Primary and secondary structure analysis was performed using the ProtParam and Self - Optimized Prediction Method with Alignment software . A phylogenetic tree was constructed from the GST proteins by the neighbor joining method using MEGA 6 . 0 to investigate the relationship among Vf GST , VvGST , and At GST proteins . To evaluate the differential expression pattern of GST genes by real - time polymerase chain reaction (PCR) , primers specific to unique regions in each gene were obtained by alignment of the sequences . Real - time PCR revealed that GST genes were expressed differentially in the leaves of V . flexuosa infected with Botrytis cinerea , E . ampelina , and Rhizobium vitis . The expression of Vf GST26625 was up - regulated , while that of others were down - regulated among five GSTs in all grapevine leaves inoculated with each pathogen . The results provided herein improve our understanding of defense responses to various pathogen attacks in grapevines .