Ambient temperature impact on hepatocellular liver damage in rats following intake of 3,4-methylenedioxymethamphetamine

Abstract

Bacground/Aim. 3,4-methylendioxymethamphetamine (MDMA, Ecstasy) is a psycho-stimulating agent. It is usualy taken orally in the form of tablets. It is absorbed throught the gastrointestinal mucous membrane. Hyperthermia is the most prominent clinical sign of MDMA intake. The most prominent forensic finding of lethal MDMA poisoning is myocardial infarction and cerebrovascular bleeding. However, liver and kidney damage: have also been described. The aim of this research was to determine if ambient temperatures affect liver damage in the experimental rats. Methods. The experiment was conducted for 8 h and 24 h, at temperatures of 12?C, 22?C and 32?C. Both biochemical parameters (ALT, AST, AP, gamma GT and LDH) and pathohistological changes of the liver were monitored. Results. Our reserch demonstrated that the most serious lever damage occurred at 32?C. Liver damage was manifested as portal inflammation, periportal necrosis, lobular necrosis, stasis, intralobular hemorrhage and incerease of liver enzymes serum activity. Conclusion. Liver damage after MDMA intake rises with the increase of ambient temperature, and it is most pronounced at the temperature of 32?C.Uvod/Cilj. Poznato je da je 3,4-metilendioksimetamfetamin (MDMA, Ecstasy) psihostimulativno sredstvo. Najcesce se unosi oralno, u vidu tableta. Resorbuje se preko sluzokoze digestivnog trakta. Najcesci klinicki znak uzimanja MDMA je hipertermija. Smrtna trovanja nakon uzimanja MDMA na obdukciji najcesce pokazuju infarkt miokarda ili cerebrovaskularno krvarenje. Medjutim, opisana su i ostecenja jetre i bubrega. Cilj ove studije bio je da se utvrdi da li temperatura okoline utice na ostecenje jetre pacova nakon primene MDMA. Metode. Eksperiment je trajao 8 i 24 h na temperaturama 12, 22 i 32?C. Praceni su biohemijski parametri (bilirubin, ALT, AST, AP, gama GT i LDH) i patohistoloske promene jetre. Rezultati. Rezultati su pokazali da su ostecenja jetre najveca na temperaturi od 32?C. Ostecenja jetre su se manifestovala kao portalna inflamacija, periportalna nekroza, lobularna nekroza, staza, intralobularno krvarenje i porast enzima jetre u krvi. Zakljucak. Ostecenje jetre nakon primene MDMA raste sa porastom temperature okoline, a najizrazenije je na temperaturi od 32?C.