Diversity and structure of a bacterial community in grassland soils disturbed by sheep grazing, in the Loess Plateau of northwestern China

Abstract

The relationship between disturbance, biodiversity, and ecosystem function has been a hot topic recently in international ecological research, and a universally applicable model remains elusive. In this study, we assessed the diversity and structure of a bacterial community in grassland soils along a disturbance gradient due to sheep grazing. Bacteria were identified based on 16S rDNA gene libraries prepared from a 12-year field experiment that included four grazing, intensity treatments: no grazing, light grazing, moderate grazing and heavy grazing in the Loess Plateau of northwestern China. We found that diversity indices of bacterial 16S rDNA increased with grazing intensity, suggesting that disturbance led to higher bacterial diversity. The bacterial community structure, measured as species composition, was also affected by grazing. In addition, the change in soil bacterial community composition was maximum under heavy grazing, based on the Sorensen similarity index. Overall, the relationship between disturbance and bacterial diversity is complex, therefore, more studies are required to determine the possibility of using microbial diversityas an indicator of ecosystem stability.