Production of packed nanospray emitters with integrated emitter-end frits for liquid-chromatography mass spectrometry

Abstract

This protocol describes how to frit the emitter-end of a pulled silica capillary and subsequently pack it to produce a robust integrated column. Integrating liquid chromatography separations directly into the emitter of a nanospray source for mass spectrometry offers improved sensitivity and resolution. This is achieved by reducing post-separation dead volume. The method described here provides an optimized setup to maximize sensitivity and separation.