Development and validation of a reverse phase high performance liquid chromatography (HPLC) method for determination of tizanidine in human plasma

Abstract

A simple and cost effective high performance liquid chromatography (HPLC) method was developed for determination of tizanidine in human plasma using liquid extraction technique. The assay of tizanidine was performed after extraction of drug from plasma using diethyl ether as extraction solvent. The isocratic elution was performed in Agilent, Zorbax SB-C 18 , 4.6 × 150 mm column maintained at 30°C with mobile phase containing acetonitrile and ammonium acetate in a ratio of 15:85 v/v, respectively. The linear relationship was found within the concentration range of 0.25 to 8 ng/ml, with a flow rate of 1 ml/min and detector wavelength of 230 nm. The evaluated validation parameters were found within the acceptable range. Use of simple HPLC technique with short retention time makes this method a convenient choice for assay of tizanidine in human plasma.