Telomere Shortening in Alveolar Macrophages of Smokers and COPD Patients~!2010-02-18~!2010-03-05~!2010-04-15~!

Abstract

Background: Tobacco smoking is reported to enhance telomere shortening in peripheral blood lymphocytes. However, telomere length of lung resident cells such as alveolar macrophages (AMs) has not been evaluated. Aim: We evaluated effect of tobacco smoking on telomere length in AMs. Methods: AMs were obtained from nine non-smokers (mean age 24.6 years, mean FEV 1 /FVC 93.8%) and ten healthy smokers (mean age 25.2 years, >10 pack-years, mean FEV 1 /FVC 90.6%). We also analyzed tissue macrophages from eight moderate to severe COPD patients (mean age 68.3 years, >10 pack-years, mean FEV 1 /FVC 54.2%, mean %pred. FEV 1 48.8%), age-matched nine healthy smokers (mean age 67.1 years, >10 pack-years, mean FEV 1 /FVC 83.7%, mean % pred. FEV 1 82.4%), and eight healthy non-smokers (mean age 71.2 years, mean FEV 1 /FVC 84.6%, mean %pred. FEV 1 84.1%). Centromere signals and telomere length were analysed using quantitative fluorescence in situ hybridization (QFISH) and confocal microscopy. Results: Telomere length was reduced in bronchoalveolar lavage macrophages of younger smokers compared with that observed in healthy non-smoker's AMs, Importantly, there was no quantitative difference in centromere signals between smokers and non-smokers. A shorter telomere length was also seen in tissue macrophages from older smokers with or without COPD compared with non-smokers. However there was no difference in telomere length between healthy smokers and smokers with COPD. Conclusion: Tobacco smoking enhances telomere shortening in AMs from patients with and without COPD.