Proteomics and bryophytes: A comparison between different methods of protein extraction to study protein synthesis in the aquatic moss leptodictyum riparium(hedw.)

Abstract

Proteomics is a leading technology for the study of proteins on a genome-wide scale, the objective of which is the large-scale identification of all protein species in a cell or tissue. In plant biology the key for successful proteomic application relies on the establishment of method to obtain high-quality protein samples. In this paper we compare three different procedures, TCA precipitation in acetone, TCA and PVPP precipitation in acetone, and precipitation in Tris-HCl to be applied for the extraction and purification of proteins from the aquatic moss Leptodic-tyum riparium. Quality gels were obtained with the Tris-HCl and TCA-acetone extraction methods. © 2007 Taylor and Francis Group, LLC.