FTIR Spectroscopy for Evaluation and Monitoring of Lipid Extraction Efficiency for Murine Liver Tissues Analysis †

Abstract

Over the past several decades, growing research on lipids and lipidomic technologies have shown the important role played by lipids in many different situations. A powerful technique used for lipids detection and characterization in biological tissues is Fourier Transform Infrared (FTIR) spectroscopy. The main goal of the present work is to exploit FTIR spectroscopy as a tool for monitoring lipid extraction efficiency by evaluating three different lipid extraction methods in murine liver tissues. In particular, infrared spectra have been obtained in the 4000–600 cm−1 wavenumber region and the contributions of different functional groups have been evidenced. The ratio values estimated using the absorbance of selected bands related to different liver constituents have been used for a quantitative comparison of the efficiency of the different extraction methods.