Abstract
In order to ascertain optimum conditions for biocatalytic processes carried out in vitro, we have designed a bio-opto-electronic system which ensures real-time compensation for depletion of adenosine triphosphate (ATP) in reactions involving transfer of phosphate groups. The system covers ATP concentration range of 2-48 μM. The report demonstrates feasibility of the device operation using apyrase as the ATP-depleting enzyme.
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CITATION STYLE
Hu, J. B., Chen, T. R., Chen, Y. C., & Urban, P. L. (2015). Microcontroller-Assisted Compensation of Adenosine Triphosphate Levels: Instrument and Method Development. Scientific Reports, 5. https://doi.org/10.1038/srep08135
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