Venous stasis-induced fibrinolysis prevents thrombosis in mice: Role of A2-antiplasmin

Abstract

Stasis of venous blood triggers deep vein thrombosis by activating coagulation, yet its effects on the fibrinolytic system are not fully understood. We examined the relationship between stasis, fibrinolysis, and the development of experimental venous thrombosis. Effects of stasis-induced deep vein thrombosis and fibrinolysis on thrombosis were examined by inferior vena cava ligation in congenic mice with and without a2-antiplasmin (a2AP), the primary inhibitor of plasmin. Venous thrombus weights were measured and thrombus composition was determined by Martius scarlet blue and immunofluorescence staining. Venous thrombi from a2AP1/1mice contained plasminogen activators, plasminogen activator inhibitor-1, plasminogen, and a2AP, which changed with thrombus age. Normal, a2AP1/1mice developed large, occlusive thrombi within 5 hours after ligation; thrombi were even larger in plasminogen-deficient mice (P < .0001) 1, 7, and 14 days after ligation. We conclude that experimental venous stasis activates the fibrinolytic system to block the development of venous thrombosis. Suppression of fibrinolysis by a2AP appears essential for stasis-induced thrombus development, which suggests that targeting a2AP may prove useful for preventing venous thrombosis.