Phytochemical analysis of ethanolic leaf extract of Cordia myxa and its anti-Inflammatory and cytotoxic activities

Abstract

OBJECTIVE: Aim: Testing Cordia myxa extract on colon cancer cell line and caspase-3 gene and COX-2 protein expression. PATIENTS AND METHODS: Materials and Methods: This study used Cordia myxa ethanolic extract at various dosages on SW480 cells. Cell proliferation was measured using MTT, also examined effect of Cordia myxa extract on caspase-3 gene expression using quantitative real-time polymerase chain reaction. The Elisa technique measured cox2 protein. RESULTS: Results: Cell proliferation tests showed the dose-dependent anticancer effects of Cordia myxa ethanolic extract. Cordia myxa extract also upregulates caspase-3 gene expression and decreases COX-2 protein levels. CONCLUSION: Conclusions: Cordia myxa may be a good choice for colon cancer research and anticancer drug development. This plant may cure cancer because of its antioxidant, anti-inflammatory, and anticancer properties.