In Vitro Studies of Sugarcane Variety Co-91017 through Micropopagation of Shoot Tip Culture

Abstract

Saccharum officinarum is an important sugarcane variety in Tamil Nadu. In our present study to optimization and standardization of protocol for induction of callus and regeneration of whole plantlets was established through in vitro culture of Sugarcane (Saccharum officinarum L.) by using shoot tip as an explant. The multiple shoot regeneration at various frequencies was observed by using different concentration and combination of growth regulators containing MS medium. The highest percentage of callus induction was observed in MS medium supplemented with 2.5mg/L, 2-4 D. The best response in terms of multiple shoot induction was observed on MS medium supplemented with BAP 2.0mg/L+IBA 0.5mg/L. When in vitro shoot lets were inoculated on to the half-strength semi solid MS medium supplemented with 2.5mg/L NAA, rooting was more profuse. Rooted shoots were transplanted in the green house for hardening and their survival rate was 85% in the field condition