Molecular engineering of photoremovable protecting groups for two-photon uncaging

Abstract

(Chemical Equation Presented) Open the cage: Symmetrical photoremovable bis-glutamate cages have been designed, synthesized, and characterized that can release a neurotransmitter with unprecedented two-photon efficiencies (see picture), up to 5 GM for BNSF-Glu (BNSF=2,7-bis-{4-nitro-8-[3-(2-propyl)-styryl] }-9,9-bis-[1-(3,6-dioxaheptyl)]-fluorene) at 800 nm, the optimal window both for tissue transparency and classically available laser sources. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.