Chemical structure of Joro spider toxin (JSTX)

Abstract

Toxic principles obtained from the venom of Joro spider (Nephila clavata) were purified with the reversed phase high performance liquid chromatography and four kinds of the major toxins (JSTX-1, -2, -3 and -4) were isolated. These principles commonly possessed 2,4-dihydroxyphenylacetyl-asparaginyl-cadaveryl moiety in the molecule. The structure of JSTX-3 was determined to be l-L-asparaginyl)-N'-[N-[4-[(3-aminopropyl)amino]butyl]β-alanyl]cadaverine. The structure activity relationship of the spider toxin was discussed.