ANALGESIC POWER OF THE INSOLUBLE FRACTION N-HEXANE OF MELINJO LEAVES (Gnetum gnemon L.) IN SWISS MICE (Mus musculus)

Abstract

Melinjo (Gnetum gnemon L.) contains various secondary metabolites, including alkaloids, flavonoids, saponins, and tannins. Flavonoids function as analgesics and inhibit cyclooxygenase enzymes, which reduce prostaglandin production and pain. This study aimed to determine the analgesic power of the n-hexane-insoluble fraction of melinjo leaves (Gnetum gnemon L.) as an effective analgesic in male white mice (Mus musculus) and to determine the most effective analgesic dose. This research was conducted using test animals of 25 male white mice (Mus musculus) divided into 5 groups, each consisting of 5 mice. The negative control group (Na-CMC) was 0.5%, the positive control group received a mefenamic acid dose of 65 kg/BW, and the insoluble fraction hexane group (ifh) received doses of 50 mg/kg BW, 100 mg/kg BW, and 200 mg/kg BW. The chemical stimulus was administered at a dose of 400 mg/kg body weight acetic acid. The number of wriggles in male white mice (Mus musculus) was measured every 10 minutes for 60 minutes. The results of the study showed a significant difference between the negative control (Na-CMC 0.5%) and various doses of insoluble fraction hexane groups, while the positive control (mefenamic acid 65 mg/kg BB) showed no difference with ifh doses of 100 mg/kg BB and 200 mg/kg BB ...