Multiplex PCR assay targeting a diguanylate cyclase-encoding gene, cgcA, to differentiate species within the genus Cronobacter

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Abstract

In a comparison to the widely used Cronobacter rpoB PCR assay, a highly specific multiplexed PCR assay based on cgcA, a diguanylate cyclase gene, that identified all of the targeted six species among 305 Cronobacter isolates was designed. This assay will be a valuable tool for identifying suspected Cronobacter isolates from food-borne investigations. © 2013, American Society for Microbiology.

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Carter, L., Lindsey, L. A., Grim, C. J., Sathyamoorthy, V., Jarvis, K. G., Gopinath, G., … Hu, L. (2013). Multiplex PCR assay targeting a diguanylate cyclase-encoding gene, cgcA, to differentiate species within the genus Cronobacter. Applied and Environmental Microbiology, 79(2), 734–737. https://doi.org/10.1128/AEM.02898-12

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