Expansion and activation kinetics of immune cells during early phase of GVHD in mouse model based on chemotherapy conditioning

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Abstract

In the present paper, we have investigated early pathophysiological events in graft-versus-host disease (GVHD), a major complication to hematopoietic stem cell transplantation (HSCT). BLLB/c female mice conditioned with busulfan/cyclophosphamide (Bu-Cy) were transplanted with allogeneic male C57BL/6. Control group consisted of syngeneic transplanted Balb/c mice. In allogeneic settings, significant expansion and maturation of donor dendritic cells (DCs) were observed at day +3, while donor T-cells CD8+ were increased at day +5 (230%) compared to syngeneic HSCT. Highest levels of inflammatory cytokines IL-2, IFN-gamma, and TNF-alfa at day +5 matched T-cell activation. Concomitantly nave T-cells gain effecr-memory phenotype and migrated from spleen to peripheral lymphoid organs. Thus, in the very early phase of GHVD following Bu-Cy conditioning donor, DCs play an important role in the activation of donor T cells. Subsequently, donor nave T-cells gain effector-memory phenotype and initiate GVHD. © 2010 Behnam Sadeghi et al.

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Sadeghi, B., Al-Hashmi, S., Hassan, Z., Rozell, B., Concha, H., Lundmark, C., … Hassan, M. (2010). Expansion and activation kinetics of immune cells during early phase of GVHD in mouse model based on chemotherapy conditioning. Clinical and Developmental Immunology, 2010. https://doi.org/10.1155/2010/142943

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