Determination of fumaric acid in commercially available malic acids and apple drinks by HPLC

Abstract

An application of HPLC to the determination of fumarie acid in commercially available malic acids and apple drinks was examined. The fumarie acid was separated on a TSK-gel 120A column (ODS C18, 4.6 mm i.d.×150mm) with an eluent of 0.1 M sodium per ~ chlorate-0.01 M sodium dihydrogenphosphate (pH 2.6). The eluate was monitored with a UV detector at 210 nm. The sensitivity of the fumarie acid was quite good and sufficient separation could be achieved within 10 min. The calibration curve between peak area and fumarie acid concentration was linear up to 2.0×10 5 M. Recoveries of fumarie acid in malic acids and apple drinks were in the range of 98 ~ 105% with good reproducibilities (R. S. D., less than 1.5%). Fumarie acid in eighteen commercially available samples (D~, L~ and DL~ malic acids and apple drinks) were determined successfully by the proposed method. © 1990, The Japan Society for Analytical Chemistry. All rights reserved.