Generation and selection of a panel of pan-filovirus single-chain antibodies using cell-free ribosome display

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Abstract

Filoviruses, which include ebolaviruses and marburgvirus, can cause outbreaks of highly lethal hemorrhagic fever. This disease causes significant morbidity and mortality in humans and non-human primates, with human fatality rates reaching 90% during some outbreaks. Currently, there is lack of licensed vaccines or antivirals for these viruses. Since early symptoms of filovirus infection mimic more common diseases, there is a strong unmet public health and biodefense need for broad-spectrum filovirus rapid diagnostics. We have generated a panel of mouse single-chain Fvantibodies (scFvs) to filovirus glycoproteins (GPs) using cell-free ribosome display and determined their cross-reactivity profiles to all known filovirus species. Two scFvs (4-2 and 22-1) were able to detect all known Ebolavirus and Marburgvirus species. This is the first report on ribosome display scFvs that can detect a broad set of filovirus GPs, which demonstrates the potential for use in diagnostics.

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APA

Kunamneni, A., Clarke, E. C., Ye, C., Bradfute, S. B., & Durvasula, R. (2019). Generation and selection of a panel of pan-filovirus single-chain antibodies using cell-free ribosome display. American Journal of Tropical Medicine and Hygiene, 101(1), 198–206. https://doi.org/10.4269/ajtmh.18-0658

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