RAPD Amplification on Cocoa (Theobroma cacao L.) from East Kolaka, Southeast Sulawesi Province

Abstract

Kolaka is one of the best quality cocoa bean producing regions in Indonesia. Plant breeding to maintain and improve crop quality needs to be done so that genetic diversity information is needed. This study aims to obtain RAPD markers that can be used to analyze cocoa genetic diversity from kolaka. A total of 4 primers from 15 selected primers produced polymorphic, bright and clear bands so that they could be used to analyze cocoa genetic diversity. The primers are OPA15, OPD3, M29, and OPP08. The highest aneling temperature was 43°C at the OPD03 primer, and the lowest aneling temperature was 36°C in the OPA15 primer.