Supplementation of Snakehead Fish Extracts into Tris Egg-Yolk Extender on Mortality and Motility of Limousin Bull Sperm

Abstract

Freezing process reduces sperm quality due to membrane damage. Antioxidants protects the membrane of spermatozoa. This study aimed to observe the effects of Snakehead Fish (Channa striata [Bloch 1793]) Extract (SFE) supplementation in tris Egg-yolk extender on mortality and motility of spermatozoa of Limousin bull (Bos primigenius f. taurus [Linnaeus, 1758]) collected from Singosari Artificial Insemination Center, Indonesia and commercial SFE. Semen from the same bull were collected and processed according to the protocol at Singosari AI Centre. Semen extender were supplemented with SFE, G0 = 0 % SFE, G1 = 2 % SFE, G2 = 4 % SFE, G3 = 6 % SFE and G4 = 8 % SFE. Each of the treatments was repeated three times. The sperm mortality and motility data from SFE treatments were analyzed using Analysis of Variance (ANOVA). The results showed no significant effect on the mortality and motility of spermatozoa (P > 0.05) amongst the treatment. However, there was reduction on mortality and tended to increase on the motility of Limousin bull spermatozoa compared to the non-supplemented extender. Furthermore, the SFE can use in eggyolk extender with some modification for the efficiency of sperm preservation.