Separation of β-Casein A1, A2, and B Using Cation-Exchange Fast Protein Liquid Chromatography

Abstract

β-Casein genetic variants A1, A2, and B were separated using cation-exchange fast protein liquid chromatography. β-Casein from a herd bulk casein sample eluted as a series of three peaks. Casein samples from individual cows containing known combinations of β-casein A1, A2, and B were used to confirm that the three peaks were β-casein genetic variants. An acid-PAGE gel confirmed the identity of the peaks that eluted from the column. © 1991, American Dairy Science Association. All rights reserved.