A METHOD FOR DETECTING AND TYPING OF SALMONELLA BY MULTIPLEX PCR

Abstract

Today in Ukraine’s market is increasing the volume of trade with livestock products. Also the number of catering services and grocery shops selling ready-made food is growing throughout the country. The veterinary service should have time to check the quality of all of these products. Only traditional bacteriological methods of isolation and identifi cation of pathogens of toxicoinfection, which is not enough in terms of increasing turnover of products, are used today. Th e one of the most dangerous toxicoinfections is salmonellosis. Typing diff erent Salmonella species gives an answer about the source of infection. Th e aim of our work was to develop a system of identifi cation of Salmonella and typing among them five serovars based on the polymerase chain reaction (multiplex PCR). We performed analysis of the nucleotide sequences of the fi ve members of the genus Salmonella, on the basis of which a primer designed for the identifi cation of any member of the genus Salmonella with simultaneous typing - Salmonella enteriса ser. Enteritidis, Salmonella enteriса ser. Typhimurium, Salmonella enterica ser. Тyphi, Salmonella enterica ser. Dublin, Salmonella enterica ser. Gallinarum- Pullorum by multiplex PCR. Th e protocol of multiplex PCR was optimization with simples positive DNA matrix.