Release of vasopressin by angiotensin II

Abstract

To test the hypothesis that angiotensin II releases antidiuretic hormone (ADH) after injection into ventricular cerebrospinal fluid, conscious adult male Sprague- Dawley rats with a lateral cerebroventricular cannula received an intraventricular injection of 0, 10, 50, or 100 ng angiotensin II. Trunk blood was collected 90 seconds later for radioimmunoassay of ADH. Plasma ADH, pg/ml (mean ± S.E.), for the four dose levels were 2.8 ± 0.7, 9.6 ± 2.5, 22.6 ± 5.6 and 25.0 ± 5.0, respectively. The increases produced by angiotensin were statistically significant (p < 0.05), suggesting a dose-response relationship. These data provide direct evidence that angiotensin releases ADH by central mechanisms. © 1975 by The Endocrine Society.