Taxonomic revision of the genus Lactarius (Russulales, Basidiomycota) in Korea

Abstract

The genus Lactarius Pers. (Russulales) is a cosmopolitan group of Basidiomycota that forms ectomycorrhizal relationships primarily with both deciduous and coniferous trees. Although the genus has been well-studied in Europe and North America, only fragmentary researches have been carried out on Asian species. In particular, the distribution of Lactarius species in South Korea is poorly understood due to insufficient morphological descriptions and a lack of DNA sequence data. In addition, the misuse of European and North American names has added to confusion regarding the taxonomy of Asian Lactarius species. In this study, the diversity of Lactarius in South Korea was evaluated by employing both morphological and phylogenetic approaches. A multi-locus phylogenetic analysis of 729 Lactarius specimens collected between 1960 and 2017 was performed using the internal transcribed spacer (ITS) region, partial nuclear ribosomal large subunit (nrLSU), partial second largest subunit of RNA polymerase II (rpb2), and minichromosome maintenance complex component 7 (mcm7). 49 Lactarius species were identified in three Lactarius subgenera: L. subg. Russularia (17 spp.), L. subg. Lactarius (22 spp.), and L. subg. Plinthogalus (10 spp.). Among them, 28 Lactarius species were identified as new to science, while just 17 were previously described Lactarius species. Four of the taxa remain un-named due to paucity of materials. A key to Korean Lactarius species, molecular phylogenies, a summary of diversity, and detailed description are provided.