Physicochemical properties, phytochemical screening, and antihyperglycemic activity of inai (Lawsonia inermis L.) leaves active fraction on streptozotocin-induced diabetic mice

Abstract

Objective: This study was designed to explore the preliminary phytochemical and physicochemical analysis of Inai (Lawsonia inermis L.) leaves and further evaluation of its antihyperglycemic effect on diabetic rats from its active fraction. Methods: The study begins with making the powder of Inai (L. inermis L.) leaves and then evaluates the physicochemical characterization. The powder of Inai leaves was fractionated and performed by the standard phytochemical screening method and streptozotocin-induced diabetic mice for evaluated the antihyperglycemic effect of Inai leaves. Result: The physicochemical evaluation showed that the powder of Inai leaves has a good and high purity level; while the phytochemical screening showed that Inai leaves fractions have various phytoconstituents. The active fraction which was obtained from ethyl acetate fraction showed a significant reduction of the blood glucose. Conclusion: The leaves of Inai (L. inermis L.) have the antihyperglycemic activity.