Solid-phase peptide synthesis of dipeptide (histidine-β-Alanine) as a chelating agent by using trityl chloride resin, for removal of Al3+, Cu2+, Hg2+ and Pb2+: Experimental and theoretical study

Abstract

Solid-phase peptide synthesis of dipeptide (histidine-β-Alanine) as a chelating agent examined by common N-9-fluorenylmethyloxycarbonyl-N-Trityl-L-histidine and tert-butyloxycarbonyl-β-Alanine-OH amino acid derivatives. Trityl chloride resin was used as a carrier resin. The molecular structure of the dipeptide was definite by using different methods such as ultraviolet visible (UV-Vis), Fourier transform infrared (FTIR), proton (1H) nuclear magnetic ressonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) and the chelating property of synthesized dipeptide was investigated for removing of metal ions Al3+, Cu2+, Hg2+ and Pb2+ in vitro. In addition, the pharmacological and biological activities of dipeptide were examined by prediction of activity spectra for substances (PASS) program.