Suscetibilidade a antifúngicos in vitro de Candida spp. Em pacientes do Hospital Universitário Regional de Maringá-PR

Abstract

Introduction: Due to the ubiquity of nosocomial yeast infections of the genus Candida spp., studies in this area have become increasingly relevant. Objective: To evaluate the antifungal Candida spp. Susceptibility profile of hospitalized patients from the Regional University Hospital of Maringá-PR (HURM). Material and Methods: The samples were submitted to broth microdilution test (MD) according to protocol M27-A3 in order to determine the minimum inhibitory concentration (MIC) and disk diffusion test according to protocol M44-A2, both from Clinical Laboratory Standards Institute (CLSI). Results and discussion: We obtained 91 isolates from urine samples, blood culture, catheter tip, endotracheal secretions, among others, from which 38 were Candida albicans, 23 were C. tropicalis, 16 were C. gabrata, 10 were C. parapsilosis, and four were C. krusei. Among the tested antifungal medications, amphotericin B, voriconazole and anidulafungin proved to be the most effective. Conclusion: In the comparison between broth microdilution and disk diffusion (DD) methods, fluconazole showed good correlation for most Candida spp., which corroborates the usefulness of DD in the screening of main antifungal agents used in clinical practice. Nonetheless, cases of resistance detected by DD need to be confirmed by MD method, which avoids false-resistant results and maximizes the treatment efficacy and reliability.