Roles of mRNA fate modulators Dhh1 and Pat1 in TNRC6-dependent gene silencing recapitulated in yeast

Abstract

Background: Animal microRNAs silence their target mRNAs by promoting mRNA degradation and inhibiting translation via GW182/TNRC6. Results: TNRC6 induces silencing effects in S. cerevisiae via CCR4-NOT complex and Dhh1-Pat1 when tethered to reporter mRNAs. Conclusion: TNRC6 utilizes the conserved mRNA fate modulators for gene silencing in yeast. Significance: Yeast genetic tools are now available to study intricate actions of TNRC6.