Propagação de mudas de helicônia em biorreator de imersão temporária

Abstract

Novel micropropagation techniques have been studied aiming the optimization of production and cost reduction. Among these micropropagation using temporarily immersion in a bioreactor is a technique that uses liquid medium in an automatzed system. This research evaluated the efficacy of a handcraft temporarily immersion system, using explants of Heliconia champneiana Griggs cv. Splash. These were submitted to three subcultures with fifteen minutes of immersion in intervals of one hour (T1), four hours (T4), six hours (T6) and eight hours (T8). Weight of the fresh mass, number of plantlets, individual weight, final volume of culture media, pH final and the overall functionality were evaluated. In evaluations between the treatments of temporarily immersion, the best performance occurred in treatment T1, being impracticable the T8 treatment for this type culture. In comparison to a conventional method (C) the treatments T1 and T4 had superior performance, demonstrating higher efficiency of the temporarily immersion for the production of plantlets. The prototype of the bioreactor of temporary immersion had good performance, presenting itself functional, of easy handling and able to be used in the study of other cultures.