Inhibition of Aphanomyces euteiches f. sp. pisi by volatiles produced by hydrolysis of Brassica napus seed meal

Abstract

Seed meal from Brassica napus (rapeseed) produced volatile fungitoxic compounds potentially of value in the control of Aphanomyces root rot of pea. Hyphal growth, germination of encysted zoospores, and oospore survival and inoculum potential, were determined in the presence of volatiles produced from B. napus seed meal. Volatile compounds from B. napus meal completely suppressed mycelial growth and germination of encysted zoospores on agar. In growth chamber bioassays, pea (Pisum sativum) seed inoculated with zoospore suspensions and incubated 24 h in the presence of volatiles from rapeseed meal had 50% lower root rot disease severity than in the absence of meal. Volatile compounds passing through soil also significantly decreased survival and inoculum potential of oospores. Gas chromatographic analysis of rapeseed tissues and the volatile compounds evolved from tissues showed that substrate glucosinolates were hydrolyzed enzymatically to produce mainly isothiocyanates. Non-autoclaved rapeseed meal produced significantly higher levels of volatile compounds than did autoclaved meal. Also, volatile compounds produced from autoclaved meal were dominated by nitriles, whereas isothiocyanates were more common volatile products from non-autoclaved meal. Our results indicate that B. napus allelochemicals responsible for toxic effects toward A. euteiches f. sp. pisi are enzymatic hydrolysis products of glucosinolates.