Biological pre -treatment use local wild strain of lignolytic filamentous bacteria to improve in vitro dry matter digestability and reduction lignin content of low quality roughages

Abstract

This study being performed at the central laboratory for graduate students Animal Resources Department/College of Agriculture/University of Baghdad/Aljadrea and in laboratory of applied microbiology Department, Food and Biotechnology Center/Directorate of Agriculture Research/Baghdad, Iraq. Wild strain of local ligninolytic bacteria of genus Streptomyces MS isolated from Iraq camel degrade feces was used to pre- treated of low quality roughages in in vitro fermentation. Roughages wheat straw (WS), corncobs (Cc), alfalfa hay (AH) was pre- treated with wild strain of genus Streptomyces MS. The results showed higher significant improvement (p < 0.01) for in vitro dry matter digestibility (IVDMD %),in vitro Organic matter digestibility (IVOMD %) for most of roughages (WS Cc, AH). Moreover, greatest (p < 0.01) improvement in(IVDMD, IVOMD %) was observed with pre-treated AH using Streptomyces MS prior of use for 21 days from 45 untreated to 67.07,67.08% treated, the smallest improving was observed (P < 0.05) with WS from 20.78 to 30.22%. This result indicated that ability of Streptomyces MS to improve (IVDMD %) was significantly (P < 0.05) affected by the roughages sources. Treated roughages with Streptomyces MS showed significant reduction in lignin content (p < 0.01).However, the highest (p < 0.01) reduction in lignin content was shown in Cc pre-treated with Streptomyces MS(from 9.8 to 6.87%), while lowest (P < 0.05)reduction in lignin content was shown in AH (from8.26 to 7.64%), These results support the possibility application of this wild strain of bacteria to improve low quality roughages in ruminants feed.