Estudo fitoquímico e avaliação da atividade moluscicida do Calophyllum brasiliense Camb (Clusiaceae)

Abstract

Recebido em 5/1/04; aceito em 17/12/04; publicado na web em 28/2/05 PHYTOCHEMICAL STUDY AND EVALUATION OF THE MOLLUSCICIDAL ACTIVITY OF Calophyllum brasiliense CAMB (CLUSIACEAE). The bioassay-guided fractionation against Biomphalaria glabrata of hydroalcoholic extracts of Calophyllum brasiliense aerial parts led to the isolation of the coumarin, named (-) mammea A/BB. The compound had its structure determined by both spectroscopic techniques (NMR 1 H, NMR 13 C, gHSQC, gHMBC and MS) and some literature comparison data. The probit analysis of (-) mammea A/BB showed LD 50 = 0.67 ppm and LD 90 = 1.47 ppm. In addition, the dichloromethane extract obtained from C. brasiliense leaves with significant molluscicidal activity against Biomphalaria glabrata was analyzed by HPLC-UV.