The Anticancer Activity of the Marine Sponge Aaptos suberitoides to Protein Profile of Fibrosarcoma Mice (Mus musculus)

Abstract

 The study was aimed to evaluating anticancer activity of A. suberitoides on protein profile of blood plasma of fibrosarcoma mice (M. musculus). Mice were induced by Benzo(a)pyrene on the subcutaneous tissue intravenously with a concentration of 0,3 gram/0,2 oleum olivarum/day for 2 weeks. Mice were treated with ethanolic extract of A. suberitoides every day for two weeks. There were six groups. The first group (I) was control. Group II, mice were induced by CMCNa, Group III mice were treated by Cyclophosphamide. Group IV, V and VI mice were treated by ethanolic extract of A. suberitoides with 500, 1000 and 1500 mg/weight. Mice blood were taken at week fifteen. Protein profile of mice was measured by electrophoresis SDS-PAGE. The protein profile indicated that the mice were induced benzo(a)pyrene (Group II, III, IV, V and VI) have a new protein band (41 kDa). The protein band not presented in control (Group I). The protein band (7 kDa) was in Groups I and II, but it not presented in Groups III, IV, V and VI. The most interesting protein band was 115 kDa, which presented only in Groups II, III and IV. The research showed that the sponge extract with concentration of 1000 and 1500 mg/weight repressed the expression of protein 115 kDa. Keywords marine sponge A. suberitoides, anticancer, fibrosarcoma, protein profile. Abstrak Penelitian ini bertujuan untuk mengetahui aktivitas antikanker dari A. suberitoides terhadap profil protein plasma darah mencit fibrosarkoma (M. musculus). Mencit diinjeksi senyawa benzo(a)piren pada jaringan subkutan secara intravena dengan konsentrasi 0,3 gram/0,2 oleum olivarum/hari selama 2 minggu. Mencit diterapi dengan ekstrak etanol A. suberitoides setiap hari selama 2 minggu. Mencit dikelompokkan menjadi 6 grup. Kelompok I adalah kontrol; Kelompok II mencit diinjeksi CMCNa; Kelompok III mencit diterapi dengan Cyclophosphamide. Kelompok IV, V, dan VI mencit diterapi dengan ekstrak etanol A. suberitoides dengan konsentrasi 500, 1000, 1500 mg/kg BB. Pada minggu ke-15 dilakukan pengambilan plasma darah mencit. Profil protein mencit dianalisis menggunakan metode SDSPAGE. Profil protein menunjukkan bahwa mencit yang diinjeksi benzo(a)pyrene (Kelompok II, III, IV, V dan VI) memiliki protein baru (41 kDa). Pita protein ini tidak muncul pada kontrol (Kelompok I). Pita protein 7 kDa terdapat pada Kelompok I dan II, tetapi tidak muncul pada Kelompok III, IV, V, dan VI. Pita protein yang paling penting adalah protein 115 kDa, yang hanya muncul pada Kelompok II, III, dan IV. Penelitian ini menunjukkan bahwa ekstrak spons dengan konsentrasi 1000 dan 1500 mg/kg BB dapat menekan ekspresi protein 115 kDa. Kata Kunci spons laut A. suberitoides, antikanker, fibrosarkoma, profil protein.