Phytochemical analysis of Urtica dioica leaves by fourier-transform infrared spectroscopy and gas chromatography-mass spectrometry

Abstract

Phytochemicals are chemical compounds formed during the plants normal metabolic processes. These chemicals are often referred to as secondary metabolites. The objective of this research was to determine the chemical composition of leaves extract from methanol. The phytochemical compound screened by gas chromatography-mass spectrometry (GC-MS) method. Fifteen bioactive phytochemical compounds were identified in the methanolic extract of Urtica dioica. The identification of phytochemical compounds is based on the peak area, retention time molecular weight, molecular formula, mass spectrometry (MS) fragment-ions and pharmacological actions. GC-MS analysis of U. dioica revealed the existence of the Oxime- methoxy-phenyl, 2, 6,-Nonadienal, 3, 7-dimethyl, 1, 2, 3- Butanetriol, Silane, triethyl(2-phenylethoxy), Benzofuran, 2,3,-dihydro, 2,5,5,8a-Tetramethyl-1,2,3,5,6,7,8, 8a-octahydronaphthalen-1-ol, 2H-Indeno[1,2-b]furan-2-one, 3,3a, 4,5,6,7,8, 8b-octahydro-8,8-dimet, 1- Dodecanamine, N, N-dimethyl, 2(3H)-Naphthalenone, 4, 4a,5,6,7,8-hexahydro-1-methoxy, D-Fructose, diethyl mercaptal, pentaacetate, [1,1-Bicyclopropyl-2-octanoic acid 2hexyl-methyl ester, Estra-1,3,5(10)- trien-17B-ol, Cyclopropaneoctanoic acid, 2-[2-pentylcycloproyl)methyl]-methyl, 1-Hydroxy-2-(2,3,4,6- tetra-O-acetyl-beta-d-glucopyranosyl)-9H-xanthe and Ethyl iso- allochlate. The FTIR analysis of U. dioica leaves proved the presence of aromatic rings, alkenes, aliphatic fluoro, alcohols, ethers, carboxlic acids, esters, nitro compounds, hydrogen bonded alcohols and phenols. It contain chemical constitutions which may be useful for various herbal formulation as anti-inflammatory, analgesic, antipyretic, cardiac tonic and antiasthamatic.